Study of the intensity of microbial contamination and nonspecific immunity under the experimental diabetes after using different ways of fixing the wound edges

Diabetes mellitus and its complications are one of the most important causes death. As a consequence hyperglycemia in patients with diabetes there is an increased risk concomitant diseases, consequences impaired ability to repair. Healing disorders result complex pathophysiology involving vascular, neuropathic, immune biochemical componentsAim research: was analyze changes inflammation markers, microbial contamination nonspecific immunity skin homogenate rats after using sutures glue.Material research methods. Experimental studies were performed on 130 white outbred adult male rats, weight from 240 320 g. induced by streptozotocin (Sigma, USA) (intraperitoneally - 65 mg / kg) previous (15 minutes) injection nicotinamide 230 kg). On background obesity, which caused 4 weeks keeping animals high-fat diet. The development 2 confirmed determining concentration glucose blood glucometer BAYER Contour Next (Germany). Animals all groups (I-IV) under thiopental anesthesia (40 kg body rats) made full-layer rectilinear incisions, cm long, anterior-lateral region abdomen. All divided into experimental groups: І group (30 – healthy wound edges sutured Vicryl 5/0 thread (ETHICON, Inc. Johnson & company (USA); ІI fixing applying glue Dermabond (USA)); ІII mellitus, thread; IV Rats Dermabond. For control, results compared those intact (10 rats). removed experiment 3, 7, 28 days surgery (90 rats).The degree opportunistic pathogenic microflora determined level urease activity Nessler's reagent. Lysozyme bacteriolytic method, as substrate suspension bacteria Micrococcus lysodeicticus.Results. obtained prove that process conditions leads significantly greater decrease immunity, animals. Also, different effects suture materials indicators protection found: larger deviations norm found where we used surgical fix edges. When comparing intensity long term (28 days) use glue, 27.3% lower lysozyme 7.0% than III sutures.Changes antimicrobial enzyme allow assess state studied tissues. Many authors note various bioliquids tissues mellitus. our significant postoperative wounds diabetes.Conclusion. Wound reliable microflora, evidenced activity, reduced characterized wound/scar tissue relation during terms observation.In remote period for closure, diabetes, used.